The Effect of BAP and IBA on In Vitro Root Cultures of  Acehnese Pomelo (Citrus maxima (Burm.) Merr.)

Ira Handayani; Laila Nazirah; Rd. Selvy Handayani

doi:10.33089/jthort.v3i1.43

The Effect of BAP and IBA on In Vitro Root Cultures of Acehnese Pomelo (Citrus maxima (Burm.) Merr.)

Ira Handayani, Laila Nazirah, Rd. Selvy Handayani

Abstract

Acehnese pomelo is considered as a potential fruit to be developed for its economic and nutritional values. However, there is a main problem in developing this fruit. Pomelo sometimes is grown from seeds, but nowadays the fruits are often found to be having lesser seeds or even seedless. Besides, it is quite challenging to grow this local pomelo not only due to the scarce of the seeds, but it also cannot be conventionally germinated. Therefore, we need to develop this fruit through tissue culture. The purpose of this study was to investigate the effect of growth regulators on germination of pomelo seeds in vitro and its organ formation from in vitro root explants seed-originated of pomelo. The study was conducted at Plant Tissue Culture Laboratory, Faculty of Agriculture, Malikussaleh University. In October 2018 to April 2019. The research was divided into two separate experiments: 1) in vitro germination of pomelo seeds and 2) in vitro organ formation from root explants from of pomelo seeds. The first experiment using Complete Randomized Design (CRD) with 10 replications. The only factor observed was the use of BAP (0 and 2 mg/l). The second experiment was the examination of plant organ formation from root explants of pomelo seeds. This experiment had 2 factors observed. The first factor was different concentrations of BAP (0, 1, and 2 mg/l). The second factor was the different concentration of IBA (0, 1.25, and 2.5 mg/l). The results indicated that the application of BAP influenced the germination of pomelo seeds. The application of 2 mg/l BAP attributed to slower growth of shoots and roots, but it demonstrated better number of shoots, roots and leaves compared to application of 0 mg/l BAP. Pamelo root explants can form adventitious shoots on media eventhough without growth regulator (BAP 0 mg/l + IBA 0 mg/l), but optimization still needs to be done in order to obtain the best concentration to initiate shoot formation.

Keywords

Roots; Auxin; Seeds; Cytokinins; Shoots;

Full Text:

PDF

References

Almeida, R. P., Nascimento, F. E., Chau, J., Prado, S. S., Tsai, C. W., Lopes, S. A., & Lopes, J. R. (2008). Genetic structure and biology of Xylella fastidiosa strains causing disease in citrus and coffee in Brazil. Appl. Environ. Microbiol., 74(12), 3690-3701.

Beyl, C. (2004). Getting Started with Tissue Culture. Plant Development and Biotechnology, 19–37.

Gray, D. J., & Trigiano, R. N. (2004). Getting started with tissue culture: media preparation, sterile technique, and laboratory equipment. In Plant Development and Biotechnology (pp. 15-37). CRC Press.

Handayani, R. S., Yunus, I., Sayuti, M., & Irawan, E. (2019). In-vitro Callus Induction of Durian (Durio zibethinus Murr.) Leaves Using Kinetin and 2, 4-D (Dichlorophenoxyacetic acid). Journal of Tropical Horticulture, 2(2), 59-64.

Handayani, R. S., Maisura, M., & Rizki, A. (2018). Pengaruh Letak Posisi Eksplan dan Sitokinin Pada Perkecambahan Biji Manggis (Garcinia mangostana L.) Lokal Aceh Secara in-Vitro. Jurnal Agrium Unimal, 14(2), 1-8.

Handayani, R. S., Poerwanto, R., Purwito, A., & Ermayanti, T. M. (2013). Pengaruh Batang Bawah dan Jenis Tunas pada Mikrografting Manggis (Garcinia mangostana) secara In Vitro. Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy), 41(1).

Hendaryono, D. P. S., &. Wijayani, A. (2004). Teknik Kultur Jaringan. Kanisius. Yogyakarta.

Isaskar, R., & Syafrial, S. (2015). Efisiensi Pemasaran Jeruk Pamelo Dalam Wilayah Magetan (Citrus grandis L. Osbek). Habitat, 22(1), 60-70.

Ismadi,, Putri, I. Y., & Hafifah, H. (2018). Characterization of Pamelo Leaves (Citrus maxima (Burm) Merr) Aceh, Indonesia. Journal of Tropical Horticulture, 1(1), 20-23.

Mandang, J. S. (2013). Media Kultur Jaringan Tanaman. Manado: Bayumedia Publishing Anggota IKAPI.

Rahayu, A. R. I. F. A. H. (2012). Karakterisasi dan Evaluasi Aksesi Pamelo (Citrus maxima (Burm) Merr.) Berbiji dan Tidak Berbiji Asli Indonesia. Disertasi Sekolah Pascasarjana, Institut Pertanian Bogor, Bogor.

Rahayu, A., Susanto, S., Purwanto, B. S., Dewi, I.S. (2012). Karakter morfologi dan kimia kultivar pamelo (Citrus maxima (Burm.) Merr.) berbiji dan tanpa biji. Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy), 40(1), 48-55.

Sofian, A. A., Prihastanti, E., & Suedy, S. W. A. (2018). Effect of IBA and BAP on Shoot Growth of Tawangmangu Tangerine (Citrus reticulate) by In-Vitro. Biosaintifika: Journal of Biology & Biology Education, 10(2), 379-387.

Srivastava, L. M. (2002). Plant Growth and Development Hormon and Envirotment. Accademic Press, London.772 p.

Féréol, L., Chovelon, V., Causse, S., Michaux-Ferriere, N., & Kahane, R. (2002). Evidence of a somatic embryogenesis process for plant regeneration in garlic (Allium sativum L.). Plant Cell Reports, 21(3), 197-203.

Susanto, S. (2004). Perubahan kualitas buah jeruk besar (Citrus grandis (L.) Osbeck) yang disimpan dan dibiarkan di pohon. Hayati, 11(1), 25.

Taiz, L. & Zeiger, E. (2002) Plant Physiology, California, The Benjamin/ Cummings Pub. Co. Inc., 559 p.

Tyas, K. N. (2012). Organogenesis dan Konservasi in vitro pamelo (Citrus maxima (Burm.) Merr). Disertasi Institut Pertanian Bogor.

Tyas, K. N., Susanto, S., Dewi, I. S., & Khumaida, N. (2016). Organogenesis tunas secara langsung pada pamelo (Citrus maxima (Burm.) Merr.). Buletin Kebun Raya, 19(1), 01-10.

DOI: http://dx.doi.org/10.33089/jthort.v3i1.43