The Success Rate of Two Explant Types of Stevia (Stevia rebaudiana Bertoni) in Various Sterilant Formulas

Riedha Kariena; Nofia Hardarani; Hilda Susanti

doi:10.33089/jthort.v3i2.57

The Success Rate of Two Explant Types of Stevia (Stevia rebaudiana Bertoni) in Various Sterilant Formulas

Riedha Kariena, Nofia Hardarani, Hilda Susanti

Abstract

This study aims to determine the effect of interaction between several sterilants and types of explants and determine the best interaction with stevia culture's success rate. This study was designed using a Factorial Complete Randomized Design with two factors and three replications. The first factor is sterilant formulas i.e: fungicide 3% + alcohol 70% + Bayclin 5%; fungicide 3% + bactericide 6% + 70% alcohol + Bayclin 5%; sublimate 0.1% + 70% alcohol + Bayclin 5%; and fungicide 3% + bactericide 6% + sublimate 0.1% + alcohol 70% + Bayclin 5%. The second factor is explant types, i.e., stevia nodes and leaves. The variables observed are the percentage of contamination (%), percentage of alive explants (%), and browning percentage. The interaction between sterilants and explant types only had a significant effect on the percentage of alive explants. The best interactions of sterilant formulas and explant types on the percentage of alive explants are 0.1% sublimate + 70% alcohol + 5% Bayclin and leaf explants.

Keywords

Stevia; In-vitro; Contamination; Tissue Culture;

Full Text:

PDF

References

Admojo, L., & Indrianto, A. (2016). Pencegahan browning fase inisasi kalus pada kultur midrib daun klon karet (Hevea brasiliensis Muell Arg) Pb 330. Indonesian Journal of Natural Rubber Research, 34(1), 25-34.

Bhatt, I. D., & Dhar, U. (2004). Factors controlling micropropagation of Myrica esculenta buch.–Ham. ex D. Don: a high value wild edible of Kumaun Himalaya. African Journal of Biotechnology, 3(10), 534-540.

Daud, N. H., Jayaraman, S., & Mohamed, R. (2012). Methods Paper: An improved surface sterilization technique for introducing leaf, nodal and seed explants of Aquilaria malaccensis from field sources into tissue culture. Aspac J. Mol Biol Biotechnol, 20, 55-58.

Djajadi, D. (2015). Pengembangan tanaman pemanis Stevia rebaudiana (Bertoni) di Indonesia. Perspektif, 13(1), 25-33.

Fauzan, Y. S. A., & Tajuddin, T. (2017). Efektivitas Merkuri Klorida (HgCl2) Pada Sterilisasi Tunas Samping Jati (Tectona grandis) In Vitro. Jurnal Bioteknologi & Biosains Indonesia (JBBI), 4(2), 78-84.

Gunawan, I. (2007). Perlakuan sterilisasi eksplan anggrek kuping gajah (Bulbophyllum beccarii Rchb. f) dalam kultur in vitro. Skripsi. Departemen Konservasi Sumberdaya Hutan dan Ekowisata Fakultas Kehutanan IPB. Bogor.

Hadiyana, A., & Syabana, M. A. (2015). Iniasi tunas secara kultur jaringan pada Stevia (Stevia rebaudiana Bertoni) dengan kosentrasi indole butyric acid (IBA) dan benzyl amino purine (BAP) yang berbeda. Jurnal Agroekoteknologi, 7(2).

Heryanto, A. F., Soegihardjo, C. J., & Purwijantiningsih, L. E. (2014). Optimalisasi Produksi Steviosida dari Kalus Daun Stevia rebaudiana Bertoni dengan Variasi Kombinasi Zat Pengatur Tumbuh Optimization of Stevioside Production from Callus Derived from Stevia rebaudiana Bertoni Leaves with Variation of Plant Growth Regulators Combination.

Hutami, S. (2016). Ulasan masalah pencoklatan pada kultur jaringan. Jurnal Agrobiogen. 4(2) : 83-88.

Oratmangun, K. M., Pandiangan, D., & Kandou, F. E. (2017). Deskripsi Jenis-Jenis Kontaminan Dari Kultur Kalus Catharanthus roseus (L.) G. Donnaman. Jurnal MIPA, 6(1), 47-52.

Putri, A. I. (2009). Kajian Glycocalyx bakteri Pada Kontaminasi Ulin (Eusideroxylon zwageri) In-Vitro. Jurnal Pemuliaan Tanaman Hutan, 3(1), 33-42.

Raini, M., & Isnawati, A. (2011). Kajian: khasiat dan keamanan stevia sebagai pemanis pengganti gula. Media Penelitian dan Pengembangan Kesehatan, 21(4).

Sinta, M. M. (2019). Pertumbuhan, Produksi Biomassa, dan Kandungan Glikosida Steviol pada Lima Klon Stevia Introduksi di Bogor, Indonesia. Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy), 47(1), 105-110.

Tabiyeh, D. T., Bernard, F., & Shacker, H. (2005, May). Investigation of glutathione, salicylic acid and GA3 effects on browning in Pistacia vera shoot tips culture. In IV International Symposium on Pistachios and Almonds 726 (pp. 201-204).

DOI: http://dx.doi.org/10.33089/jthort.v3i2.57