In-vitro Callus Induction of Durian (Durio zibethinus Murr.) Leaves Using Kinetin and 2,4-D (Dichlorophenoxyacetic acid)

Rd. Selvy Handayani, Ismadi Yunus, M. Sayuti, Endri Irawan

Abstract


Durian (Durio zibethinus Murr.) is a tropical fruit grown in Southeast Asia and it has high nutritional and economic values and highly appreciated by consumers in Indonesia.  Unfortunately, there are several obstacles in planting durian in Indonesia, specifically in Aceh.  One of the abstacles is the scarcity of superior durian plants. Generally, plant conservations done conventionally through vegetative propagation.  However, this technique has disadvantage such as harming the initial plants.    Therefore, plant culture tissue has been used as a modern technique to develop durian plants. The objective of this research was to evaluate the effect of kinetin and 2,4 D on development of durian leaf explants through in-vitro bioassay. The research was conducted at Tissue Culture Laboratory, Faculty of Agriculture, Malikussaleh University from January to April 2019 using Completely Randomized Design (CRD) Factorial with 2 factors observed. The first factor was Kinetin (K): 0.0, 0.1 and 0.5 ppm. The second factor was auxin 2,4 D: 0.0, 0.5 and 1.0 ppm.  The results showed that the in-vitro application of kinetin affected the induction of leaf callus.  The application of 0.5 ppm of kinetin gave the best result compared to others.  The in-vitro application of 2,4 D 0.5 ppm also possessed the best result compared to others. There was no interaction beween in-vitro application of kinetin and 2,4 D on the growth of durian leaf callus at all parameters observed.


Keywords


Auxin, Explants, Morphogenesis, Genetic resources, Cytokinin

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References


Anjani, N. P., (2011). Sterilization techniques and growth response of floral stalk explants of orchid Phaleonopsis sp. treated with growth regulator 2i-p in vitro (written in Indonesian language). Script. Program Sarjana, Universitas Udayana Denpasar, Indonesia.

Ariati, S. N., Muslimin, Waeniati, Suwastika dan Nengah. (2012). Induction of cocoa (Theobroma cacao L.) on MS medium treated with 2,4-D, BAP and coconut water (written in Indonesian language). Jurnal Natural Science, 1(1).74-84.

Chugh, S., Satyakam, G and I. Usha. R. (2009). Micropropagation of orchids: A review on the potential of different explants. Scientia Horticulturae, 122 (4): 507-520.

Daud, N. H., Jayaraman, S., dan Mohamed, R. (2012). Methods Paper: An Improved Surface Sterilization Technique for Introducing Leaf, Nodal and Seed Explants of Aquilaria Malaccensis from Field Sources Into Tissue Culture. Aspac J. Mol Biol Biotechnol 20. 55-58.

[Deptan] Departemen Pertanian. (2012). Pertanian. http://hortikultura.deptan.go. id/? q=node/327. [12 Maret 2013].

Fatmawati, A. (2008). Study of Concentrations of BAP and 2,4-D on Callus Induction of Artemisia annua L. In Vitro (written in Indonesian language). Script. Fakultas Pertanian UNS. Surakarta.

Feng, J., Wang, Y., Yi, X., Yang, W., and HAE, X. 2016. Phenolics from Durian Exert Pronounced NO Inhibitory and Antioxidant Activities. Journal of Agricultural and Food Chemistry. 64(21). 4273-4279.

Handayani, RS., Poerwanto, R., Sobir, Purwito, A., Ermayanti, TM. (2013). Effect of rootstock and types of shoots on micrografting in mangosteen in vitro (written in Indonesian language). J. Agron. Indonesia. 41 (1) : 47-53

Handayani, RS., Ismadi, Sayuti, M., Hasyim, CR. (2018). Effect of sterilant ethanol and mercuric chloride on the growth of durian (Durio zibethinus Murr.) shoot explants in vitro (written in Indonesian language). Prosiding Forum Komunikasi Perguruan Tinggi Pertanian Indonesia (FKPTPI) Banda Aceh: 271-276.

Indah, N. P., dan Ermavitalini, D. (2013). Callus induction of tamanu (Calophyllum inophyllum Linn.) leaves with combination of concentrations of 6-Benzylanimopurine (BAP) and 2,4-Dicholoropeoxyacetic Acid (2,4-D) (written in Indonesian language). Jurnal Sains dan Seni Pomits, 2 (1). 2337-3520.

Lestari, E. G., dan Yunita, R. (2008). Propagation of Artemisia annua L. in vitro. (written in Indonesian language). Jurnal Agrobiogen, 4 (1). 41- 44.

Marlin, Yulian, dan Hermansyah. (2012). Initiation of embryogenic callus on cultured banana bud in Curup with application of sucrose, BAP and 2,4 D (written in Indonesian language). J., Agrivor 11(2). 275-283.

Mekonnen T, Diro M, Sharma M. (2013). Analternative safer and cost effective surface sterilization method for sugarcane (Saccharum officinarum L.) explants. Afr J Biol, 1(1), 28-32.

Muliati, T. Nurhidayah, dan Nurbaiti. (2017). Effect of NAA, BAP and their combinations on MS media to the development of Sansevieria macrophylla explants in vitro (written in Indonesian language). JOM FAPERTA, 4 (1). 1-13.

Rahayu, T. dan U. Mardini. (2015). Response of nodal and leaf explants of Madeira-vine (Anredera cordifolia L.) on MS media with varied concentrations of BAP (written in Indonesian language). Seminar Nasional XII Pendidikan Biologi FKIP UNS. Surakarta: Universitas Negeri Surakarta.

Riyadi, I., dan Tirtoboma. (2004). Effect of application of on somatic embryogenesis induction of Arabica coffee (written in Indonesian language). Balai Penelitian Bioteknologi Perkebunan Indonesia. Buletin Plasma Nutfah. Bogor. 10 (2). 82-89.

Santoso, U., dan Fatimah. (2014). Kultur Jaringan Tanaman. Malang : UMM Press.

Sitinjak, M. A., Isda, M. N., dan Fatonah, S. (2015). Callus induction of rodent tuber (Typhonium sp.) leaf explants associated with application of 2,4-D and Kinetin (written in Indonesian language). Jurnal Biologi, 8(1).38.

Sugiono, C., dan Hasbianto, A. (2014). The development of tissue culture technique application on potato plants (Solanum tuberosum L.) (written in Indonesian language) In Prosiding Seminar Nasional “ Inovasi Teknologi Pertanian Spesifik Lokasi” Banjarbaru 6-7 Agusutus 2014. (Pp 435-443).

Sugiyarto, L., dan P. C. Kuswandi. (2013). Exploration of sterilization method and types of media for durian (Durio zibethinus, L.) propagation in vitro (written in Indonesian language). J. Sains Dasar. 2(1). 20-24.

Teguh, S. M. (2017). Effect of growth regulators 2,4-D (Dichlorophen-oxyacetid Acid) and kinetin to callus induction of cinnamon (Cinnamomun burmanii) leaf explants (written in Indonesian language). Jambi. Universitas Jambi.

Widyawati, G. (2010). Effect of Several Concentrations of NAA and BAP to callus induction of bubble bush (Jatropha curcas). Thesis. Surakarta. Universitas Sebelas Maret.

Yunus, A., Samanhudi., A.T. Sakya dan M. Rahayu. (2010). Teknologi Kultur Jaringan. Surakarta: UNS Press.

Zulkarnain dan Lizawati. (2011). Callus proliferation of hypocotyl and cotyledon explants of bubble bush (Jatropha curcas L.) treated with 2,4-D (written in Indonesian language). Jurnal Natural Indonesia, 14 (1). 19-25.

Zulkarnain, H. (2009). Kultur Jaringan Tanaman. Bumi Aksara. Jakarta.




DOI: http://dx.doi.org/10.33089/jthort.v2i2.23

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